UCLAiGEM:Notebook/MaterialsProcessing/2015/04/06

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<html><head><title>6 April 2015</title><meta content="text/html; charset=UTF-8" http-equiv="content-type"><style type="text/css">.lst-kix_bb15pfunbzv3-8>li:before{content:"\0025a0 "}.lst-kix_bb15pfunbzv3-4>li:before{content:"\0025cb "}.lst-kix_bb15pfunbzv3-6>li:before{content:"\0025cf "}.lst-kix_bb15pfunbzv3-1>li:before{content:"\0025cb "}ul.lst-kix_bb15pfunbzv3-0{list-style-type:none}.lst-kix_bb15pfunbzv3-7>li:before{content:"\0025cb "}.lst-kix_bb15pfunbzv3-5>li:before{content:"\0025a0 "}ul.lst-kix_bb15pfunbzv3-7{list-style-type:none}ul.lst-kix_bb15pfunbzv3-8{list-style-type:none}.lst-kix_bb15pfunbzv3-2>li:before{content:"\0025a0 "}ul.lst-kix_bb15pfunbzv3-5{list-style-type:none}ul.lst-kix_bb15pfunbzv3-6{list-style-type:none}.lst-kix_bb15pfunbzv3-0>li:before{content:"\0025cf "}ul.lst-kix_bb15pfunbzv3-3{list-style-type:none}.lst-kix_bb15pfunbzv3-3>li:before{content:"\0025cf "}ul.lst-kix_bb15pfunbzv3-4{list-style-type:none}ul.lst-kix_bb15pfunbzv3-1{list-style-type:none}ul.lst-kix_bb15pfunbzv3-2{list-style-type:none}ol{margin:0;padding:0}.c2{padding-left:0pt;widows:2;orphans:2;direction:ltr;margin-left:72pt}.c0{padding-left:0pt;widows:2;orphans:2;direction:ltr;margin-left:36pt}.c4{widows:2;orphans:2;direction:ltr}.c1{max-width:468pt;background-color:#ffffff;padding:72pt 72pt 72pt 72pt}.c3{margin:0;padding:0}.c5{font-size:20pt}.title{widows:2;padding-top:0pt;line-height:1.15;orphans:2;text-align:left;color:#000000;font-size:21pt;font-family:"Trebuchet MS";padding-bottom:0pt;page-break-after:avoid}.subtitle{widows:2;padding-top:0pt;line-height:1.15;orphans:2;text-align:left;color:#666666;font-style:italic;font-size:13pt;font-family:"Trebuchet MS";padding-bottom:10pt;page-break-after:avoid}li{color:#000000;font-size:11pt;font-family:"Arial"}p{color:#000000;font-size:11pt;margin:0;font-family:"Arial"}h1{widows:2;padding-top:10pt;line-height:1.15;orphans:2;text-align:left;color:#000000;font-size:16pt;font-family:"Trebuchet MS";padding-bottom:0pt;page-break-after:avoid}h2{widows:2;padding-top:10pt;line-height:1.15;orphans:2;text-align:left;color:#000000;font-size:13pt;font-family:"Trebuchet MS";font-weight:bold;padding-bottom:0pt;page-break-after:avoid}h3{widows:2;padding-top:8pt;line-height:1.15;orphans:2;text-align:left;color:#666666;font-size:12pt;font-family:"Trebuchet MS";font-weight:bold;padding-bottom:0pt;page-break-after:avoid}h4{widows:2;padding-top:8pt;line-height:1.15;orphans:2;text-align:left;color:#666666;font-size:11pt;text-decoration:underline;font-family:"Trebuchet MS";padding-bottom:0pt;page-break-after:avoid}h5{widows:2;padding-top:8pt;line-height:1.15;orphans:2;text-align:left;color:#666666;font-size:11pt;font-family:"Trebuchet MS";padding-bottom:0pt;page-break-after:avoid}h6{widows:2;padding-top:8pt;line-height:1.15;orphans:2;text-align:left;color:#666666;font-style:italic;font-size:11pt;font-family:"Trebuchet MS";padding-bottom:0pt;page-break-after:avoid}</style></head><body class="c1"><p class="c4"><span class="c5">Silk Degumming</span></p><ul class="c3 lst-kix_bb15pfunbzv3-0 start"><li class="c0"><span><a href = "http://openwetware.org/wiki/UCLAiGEM:Protocols/degumming">Following Kaplan 2011 solubilization protocol</a></span></li><li class="c0"><span>Used 1.646 g of silk, instead of 5 g</span></li><li class="c0"><span>used 1 L of 0.02 M sodium carbonate instead of 2 L (2.12 g in 1L)</span></li><li class="c0"><span>note: boiling 1L water on a hot plate takes a long time (1 hr)</span></li></ul><ul class="c3 lst-kix_bb15pfunbzv3-1 start"><li class="c2"><span>added silk while water was boiling softly (tiny bubbles forming)</span></li></ul><ul class="c3 lst-kix_bb15pfunbzv3-0"><li class="c0"><span>boil exactly for 30 min</span></li></ul></body></html>