UCLAiGEM:Notebook/JulianLutze/2015/02/04

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Ligation

Completed Ligation for the tamura sequence. Everything was pre-digested from last week. The components were verified/purified on 2/2. Used 2.5ul digested vector (20ng/ul) and 7.3ul digested insert (16ng/uL). Followed protocol.

Tamura Sequence
Material Amount (uL)
Vector 2.5
Insert 7.3
Buffer 2
ligase 1
water 7.2