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Ligation of Tamura Insert and psb1A3 Vector

  • After gel purification of PCR products, a ligation of Tamura insert and psb1A3 vector was conducted.
Material Amount (μL)
psb1A3 Vector 2.5 μL
Tamura Insert 7.3 μL
Buffer 2 μL
Ligase 1 μL
Milli-Q Water 7.2 μL

The contents were pipetted into a vial and ligation was allowed to happen at room temperature for 20 minutes.