Tk:wanted parts

From OpenWetWare
Jump to: navigation, search
  • Expression biobrick plasmids
    • T7 promoter
    • T3
    • SP6
    • T7 RNA polymerase containing strains
    • also T3, SP6 What are the patent issues on these?
  • xre protein, promoters (subtilis)
  • antibiotic cassettes
    • Apramycin
    • Trimethoprim
  • Transposase genes
  • R6 origin plasmids
  • chomosomal integration fragments
    • choice of locations on the chromosome
  • cell killing enzymes
    • VSVG
  • protein export tags
  • protein cleavage domains
  • protein purification tags, antibody epitopes
    • FLAG
    • c-MYC
      • EQKLISEEDL
    • HA
      • YPYDVPDYA
    • 6HIS
      • HHHHHH
    • Strep
      • AWRHPQFGG
      • Ala-Trp-Arg-His-Pro-Gln-Phe-Gly-Gly
    • GST (n-terminal)
    • Chitin binding
    • BCCP biotin carboxyl carrier protein
      • PMID: 14736427
      • PMID: 10211839
      • PMID: 10470036
    • VSVG
    • Thrombin cleavage domain
      • LVPRGS (Leu-Val-Pro-Arg-Gly-Ser), factor Xa cleaved
    • Calmodulin tag
    • S-tag
    • GFP protein tags


  • protein solubility
    • maltose binding protein
    • Thioredoxin
  • protein splicing domains (inteins)
  • surface display proteins
    • LPP/OmpA
    • Neisseria IgA1
  • New fluorescent proteins
    • EBFP2 blue
    • new red monomers
  • luminescent reporters
    • renilla
    • photuris
    • bacterial (Photorhabdus)
    • dual reporter renilla/photuris being done with John Cumbers (Brown)
    • dual reporter red/green beetle luciferase dual color
  • enzymatic reporters
    • HRP protein
    • lacZ
    • Alkaline phosphatase
  • Zinc finger domains
  • Repair enzymes
    • UDG
    • mutS, mutC
  • RNA sensor domains for all small molecules
  • negative selection markers
    • sacB
    • pheS mutated
  • toxic proteins
    • ccdB and similar
  • Mesoplasma florum genes
    • recT, recE
    • GFP