Kubke Lab:Protocols/CVStainTable
From OpenWetWare
(Redirected from TemplateKubkeLabCVStain)
Cresyl Violet staining
For more informattion see Kubke_Lab:Nissl_Stain_Protocol
| Date | ||
| Defatting and rehydration step | ||
| Solution | Time | Comments |
| Water | ||
| 75% alcohol | ||
| 95% alcohol | ||
| 100% alcohol 1 | ||
| 100% alcohol 2 | ||
| Xylene 1 | ||
| Xylene 2 | ||
| Xylene 3 | ||
| Xylene 2 | ||
| Xylene 1 | ||
| 100% alcohol 2 | ||
| 100% alcohol 1 | ||
| 95% alcohol | ||
| 75% alcohol | ||
| Water | ||
| Staining and differentiation step | ||
| Solution | Time | Comments |
| Water | ||
| Cresyl Violet | ||
| 50% alcohol | ||
| 70% alcohol acetic acid | ||
| 95% alcohol | ||
| 100% alcohol 1 | ||
| 100% alcohol 2 | ||
| Xylene 1 | ||
| Xylene 2 | ||
| Xylene 3 | ||
| Coverslip | ||