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Maxiprep of plasmid DNA from E. coli: Difference between revisions
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Maxiprep of plasmid DNA from E. coli (view source)
Revision as of 06:45, 18 February 2007
, 18 February 2007→Ingredients
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* 1mL TEG (25mM Tris-Cl, 10mM EDTA, 50mM dextrose) | * 1mL TEG (25mM Tris-Cl, 10mM EDTA, 50mM dextrose) | ||
* 111μL 20mg/mL lysozyme | * 111μL 20mg/mL lysozyme | ||
* 2mL worth of components for solution 2: 200μL 10% SDS, 100μL 4M NaOH, 1.7 | * 2mL worth of components for solution 2: 200μL 10% SDS, 100μL 4M NaOH, 1.7 mL autoclaved water | ||
* 1.5mL Solution 3: 3M K<sup>+</sup>, 5M acetate | * 1.5mL Solution 3: 3M K<sup>+</sup>, 5M acetate | ||
* 3.7mL isopropanol | * 3.7mL isopropanol | ||
