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Ampicillin: Difference between revisions
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The authors reccommend not allowing cultures to reach stationary phase if you need a high proportion of cells to contain your plasmid. | The authors reccommend not allowing cultures to reach stationary phase if you need a high proportion of cells to contain your plasmid. | ||
Typical concentrations of ampicillin are 50 ug/ml for low copy plasmids and 100 ug/ml for high copy plasmids. Stock solutions are typically at 100 mg/ml, so that 1 ml of antibiotic can be added to 1 liter of broth or agar. Stock solutions made in 50% alcohol remain liquid at -20 C and are easy to pipet. Cool agar to 55C or below prior to adding antibiotic. | |||
==References== | ==References== | ||
Molecular Cloning: A Laboratory Manual, Vol 1. | Molecular Cloning: A Laboratory Manual, Vol 1. | ||
