3,675
edits
Ampicillin: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
m
Nice image! some minor formatting changes
Barry Canton (talk | contribs) m (Nice image! some minor formatting changes) |
|||
| Line 3: | Line 3: | ||
==Mechanism of Resistance== | ==Mechanism of Resistance== | ||
Expression of [[β-lactamase]] neutralizes ampicillin. When this enzyme is expressed on a high-copy number plasmid there is significant diffusion into the extracellular medium. As a result non-resistant satellite colonies may form around larger resistant colonies. | Expression of [[β-lactamase]] neutralizes ampicillin. When this enzyme is expressed on a high-copy number plasmid there is significant diffusion into the extracellular medium. As a result non-resistant satellite colonies may form around larger resistant colonies. | ||
[[Image:Amp2.jpg|thumb|300px|left|Satellite colonies on an Ampicillin plate]] | |||
<br style="clear:both" /> | |||
==Usage Notes== | ==Usage Notes== | ||
| Line 12: | Line 14: | ||
The authors reccommend not allowing cultures to reach stationary phase if you need a high proportion of cells to contain your plasmid. | The authors reccommend not allowing cultures to reach stationary phase if you need a high proportion of cells to contain your plasmid. | ||
==References== | ==References== | ||
Molecular Cloning: A Laboratory Manual, Vol 1. | Molecular Cloning: A Laboratory Manual, Vol 1. | ||
