1,973
edits
TOP10 chemically competent cells: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
TOP10 chemically competent cells (view source)
Revision as of 22:52, 8 November 2006
, 8 November 2006→CCMB80 buffer
| Line 44: | Line 44: | ||
* adjust pH DOWN to 6.4 with 0.1N HCl if necessary | * adjust pH DOWN to 6.4 with 0.1N HCl if necessary | ||
** adjusting pH up will precipitate manganese dioxide from Mn containing solutions. | ** adjusting pH up will precipitate manganese dioxide from Mn containing solutions. | ||
* sterile filter and store at 4C | * sterile filter and store at 4C | ||
* slight dark precipitate appears not to affect its function | |||
===Measurement of competence=== | ===Measurement of competence=== | ||
