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TOP10 chemically competent cells: Difference between revisions
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TOP10 chemically competent cells (view source)
Revision as of 22:49, 8 November 2006
, 8 November 2006→Preparing competent cells
| Line 32: | Line 32: | ||
* Store at -80C indefinitely. | * Store at -80C indefinitely. | ||
** Flash freezing does not appear to be necessary | ** Flash freezing does not appear to be necessary | ||
* Test competence (see below) | |||
** Good cells should yield around 100 colonies | |||
===CCMB80 buffer=== | ===CCMB80 buffer=== | ||
