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BME100 f2014:Group29 L4: Difference between revisions
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→Q1. What is the function of each component of PCR?
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=RESEARCH AND DEVELOPMENT= | =RESEARCH AND DEVELOPMENT= | ||
==Q1. What is the function of each component of PCR?== | ==Q1. What is the function of each component of PCR?== | ||
'''Template DNA:''' A strand that is used as the DNA wanting to be copied | '''Template DNA:''' A strand that is used as the DNA wanting to be copied, can be thought of as a "mold" or "template" (http://en.mimi.hu/biology/template.html). | ||
'''Primers:''' Copy specific DNA sequences by attaching to either end | '''Primers:''' Copy specific DNA sequences by attaching to either end (http://en.wikipedia.org/wiki/Primer_(molecular_biology)). | ||
'''Taq Polymerase:''' An enzyme typically found in hot springs, it can stand temperatures between 70 and 75 degrees centigrade (http://www.taqpolymerase.org/). | '''Taq Polymerase:''' An enzyme typically found in hot springs, it can stand temperatures between 70 and 75 degrees centigrade, its most common use is PCR (http://www.taqpolymerase.org/). | ||
'''Deoxyribonucleotides (dNTP’s):''' | '''Deoxyribonucleotides (dNTP’s):''' A nucleotide that is bonded to deoxyribose that is then bonded to a phosphate (http://medical-dictionary.thefreedictionary.com/Deoxyribonucleotides). | ||
==Q2. What happens to the components (listed above) during each step of thermal cycling?== | ==Q2. What happens to the components (listed above) during each step of thermal cycling?== | ||
