BME100 f2014:Group29 L4: Difference between revisions
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| [[Image:BME103student.jpg|100px|thumb|Zied Alghamdi]] | | [[Image:BME103student.jpg|100px|thumb|Zied Alghamdi]] | ||
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=PCR Lab A= | |||
==Materials== | ==Materials== | ||
Revision as of 02:05, 28 October 2014
PCR Lab A
OUR TEAM
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PCR Lab A
Materials
- Lab coat and disposable gloves
- PCR reaction mix, 8 tubes, 50 μL containing Taq DNA polymerase, MgCl2, and dNTP's
- DNA/primer mix, 50 μl each: all have the same forward and revers primer but different templates of DNA
- A strip of empty PCR tubes
- Disposable pipette tips: only use each only once. Never re-‐use disposable pipette tips or samples will be cross-‐contaminated
- Cup for discarded tips
- Micropipettor
- OpenPCR machine
PCR Reaction Sample List
| Tube Label | PCR Reaction Sample | Patient ID |
| G29 + | Positive control | none |
| G29 - | Negative control | none |
| G29 1-1 | Patient 1, replicate 1 | |
| G29 1-2 | Patient 1, replicate 2 | |
| G29 1-3 | Patient 1, replicate 3 | |
| G29 2-1 | Patient 2, replicate 1 | |
| G29 2-2 | Patient 2, replicate 2 | |
| G29 2-3 | Patient 2, replicate 3 |
OpenPCR Program
HEATED LID: 100°C
INITIAL STEP: 95°C for 2 minutes NUMBER OF CYCLES: 35
Denature at 95°C for 30 seconds, Anneal at 57°C for 30 seconds, and Extend at 72°C for 30 seconds
FINAL STEP: 72°C for 2 minutes FINAL HOLD: 4°C
