943
edits
Biomod/2014/ASU/Results: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
no edit summary
No edit summary |
No edit summary |
||
| Line 1,588: | Line 1,588: | ||
<p class = "serif" style = "color: #000000; font-size: 25px;"><span class = "mw-headline">Positive Control DNA Antibody Binding to Alpha-Thrombin</span></p> | <p class = "serif" style = "color: #000000; font-size: 25px;"><span class = "mw-headline">Positive Control DNA Antibody Binding to Alpha-Thrombin</span></p> | ||
<hr> | <hr> | ||
<p style = "color: #000000; font-size: 16px;">Positive control DNA antibodies were constructed containing the aptamers with known sequences which had previously been shown to specifically bind alpha-thrombin protein at different | <p style = "color: #000000; font-size: 16px;">Positive control DNA antibodies were constructed containing the aptamers with known sequences which had previously been shown to specifically bind alpha-thrombin protein at different sites(1). For simplicity, these aptamers were labeled “aptamer A” and “aptamer B”. The DNA antibodies were created using the tile assembly protocol under the Protocols tab of the Experiment section. The sequence for the control aptamers are listed in Table E1 under the Materials tab.</p> | ||
<p style = "color: #000000; font-size: 16px;">The DNA antibodies were created to incorporate known the control aptamers in different positions on the tile. The number of aptamers and their orientation with respect to each other were also changed as illustrated in the schematic figure R1 below. Some of the DNA antibodies include a filler strand instead of an aptamer strand. This filler strand is made up of an oligo which only contains the sequence for the insertion into the tile such that when it binds, it “fills” the tile to be two dimensional.</p><br> | <p style = "color: #000000; font-size: 16px;">The DNA antibodies were created to incorporate known the control aptamers in different positions on the tile. The number of aptamers and their orientation with respect to each other were also changed as illustrated in the schematic figure R1 below. Some of the DNA antibodies include a filler strand instead of an aptamer strand. This filler strand is made up of an oligo which only contains the sequence for the insertion into the tile such that when it binds, it “fills” the tile to be two dimensional.</p><br> | ||
<img src = "http://openwetware.org/images/0/08/Screen_Shot_2014-10-25_at_8.08.37_PM.png"><br> | <img src = "http://openwetware.org/images/0/08/Screen_Shot_2014-10-25_at_8.08.37_PM.png"><br> | ||
