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Biomod/2014/ASU/Results: Difference between revisions
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<p style = "color: #000000; font-size: 16px;">The DNA antibodies were created to incorporate known the control aptamers in different positions on the tile. The number of aptamers and their orientation with respect to each other were also changed as illustrated in the schematic figure R1 below. Some of the DNA antibodies include a filler strand instead of an aptamer strand. This filler strand is made up of an oligo which only contains the sequence for the insertion into the tile such that when it binds, it “fills” the tile to be two dimensional.</p><br> | <p style = "color: #000000; font-size: 16px;">The DNA antibodies were created to incorporate known the control aptamers in different positions on the tile. The number of aptamers and their orientation with respect to each other were also changed as illustrated in the schematic figure R1 below. Some of the DNA antibodies include a filler strand instead of an aptamer strand. This filler strand is made up of an oligo which only contains the sequence for the insertion into the tile such that when it binds, it “fills” the tile to be two dimensional.</p><br> | ||
<img src = "http://openwetware.org/images/0/08/Screen_Shot_2014-10-25_at_8.08.37_PM.png"><br> | <img src = "http://openwetware.org/images/0/08/Screen_Shot_2014-10-25_at_8.08.37_PM.png"><br> | ||
<p style = "color: #000000; font-size: 16px;">Positive control DNA antibodies were incubated at room temperature with alpha-thrombin and then were run on an 8% Native PAGE and imaged. This process is known as an Electrophoretic Mobility Shift Assay (EMSA).</p | <p style = "color: #000000; font-size: 16px;">Positive control DNA antibodies were incubated at room temperature with alpha-thrombin and then were run on an 8% Native PAGE and imaged. This process is known as an Electrophoretic Mobility Shift Assay (EMSA).</p> | ||
<p style = "color: #000000; font-size: 16px;">There was an obvious shift in the gel bands between the lanes containing only DNA antibody and those containing DNA antibody incubated with protein. This indicates that the protein is binding the DNA antibodies which alters the progression through the gel. These shifts are seen for all of the positive control DNA antibodies indicating they all bound protein. </p><br> | <p style = "color: #000000; font-size: 16px;">There was an obvious shift in the gel bands between the lanes containing only DNA antibody and those containing DNA antibody incubated with protein. This indicates that the protein is binding the DNA antibodies which alters the progression through the gel. These shifts are seen for all of the positive control DNA antibodies indicating they all bound protein. </p><br> | ||
<img src = "http://openwetware.org/images/d/da/Screen_Shot_2014-10-25_at_8.12.28_PM.png"><br> | <img src = "http://openwetware.org/images/d/da/Screen_Shot_2014-10-25_at_8.12.28_PM.png"><br> | ||
