943
edits
Biomod/2014/ASU/Results: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
no edit summary
No edit summary |
No edit summary |
||
| Line 1,582: | Line 1,582: | ||
<p class = "serif" style = "color: #000000; font-size: 25px;"><span class = "mw-headline">Testing Tile</span></p> | <p class = "serif" style = "color: #000000; font-size: 25px;"><span class = "mw-headline">Testing Tile</span></p> | ||
<hr> | <hr> | ||
<p style = "color: #000000; font-size: 16px;">As a form of positive control, we created tiles of our basic design that could incorporate four random aptamers to specifically incorporate two aptamers known to bind alpha thrombin, called “aptamer A” and “aptamer B”, in specific configurations (shown below). The tiles were created using the tile assembly protocol under the Protocols tab of the Experiment section.</p> | <p style = "color: #000000; font-size: 16px;">As a form of positive control, we created tiles of our basic design that could incorporate four random aptamers to specifically incorporate two aptamers known to bind alpha thrombin, called “aptamer A” and “aptamer B”, in specific configurations (shown below). The tiles were created using the tile assembly protocol under the Protocols tab of the Experiment section.</p><br> | ||
<img src = "http://openwetware.org/images/8/8c/Screen_Shot_2014-10-23_at_4.05.45_AM.png"> | <img src = "http://openwetware.org/images/8/8c/Screen_Shot_2014-10-23_at_4.05.45_AM.png"> | ||
<img src = "http://openwetware.org/images/c/c3/Screen_Shot_2014-10-23_at_4.06.24_AM.png"> | <img src = "http://openwetware.org/images/c/c3/Screen_Shot_2014-10-23_at_4.06.24_AM.png"> | ||
| Line 1,593: | Line 1,593: | ||
<p style = "color: #000000; font-size: 16px;">The double nature of the bands in AT2R’ is characteristic of tiles of this nature: another previously-made tile, AT2R -- which incorporated two randomized oligonucleotides into the opposite sites as AT2R’ -- also showed such a signal in the control lanes. We suspect that this phenomenon is occurring due to some tiles failing to incorporate the aptamer strands. This should not be an issue, however, as the remaining tiles which have the inserts incorporated appear to be present in approximately equal amount, which should provide in itself a large enough selectable pool. Nonetheless, we are continuing to investigate the cause of the phenomenon and how it might possibly be prevented.</p> | <p style = "color: #000000; font-size: 16px;">The double nature of the bands in AT2R’ is characteristic of tiles of this nature: another previously-made tile, AT2R -- which incorporated two randomized oligonucleotides into the opposite sites as AT2R’ -- also showed such a signal in the control lanes. We suspect that this phenomenon is occurring due to some tiles failing to incorporate the aptamer strands. This should not be an issue, however, as the remaining tiles which have the inserts incorporated appear to be present in approximately equal amount, which should provide in itself a large enough selectable pool. Nonetheless, we are continuing to investigate the cause of the phenomenon and how it might possibly be prevented.</p> | ||
<img src = "http://openwetware.org/wiki/Image:Screen_Shot_2014-10-23_at_4.13.19_AM.png"> | <img src = "http://openwetware.org/wiki/Image:Screen_Shot_2014-10-23_at_4.13.19_AM.png"> | ||
</div> | |||
