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Isolation of murine splenocytes: Difference between revisions
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Isolation of murine splenocytes (view source)
Revision as of 09:28, 19 October 2006
, 19 October 2006→Protocol B
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#Homogenize the spleen between the frosted ends of the slides. | #Homogenize the spleen between the frosted ends of the slides. | ||
#Pass the homogenized spleen through the cell strainer (or nylon mesh) mounted on a 50mL conical. | #Pass the homogenized spleen through the cell strainer (or nylon mesh) mounted on a 50mL conical. | ||
#Continue with step 4 of Procedure | #Continue with step 4 of Procedure A. | ||
==Notes== | ==Notes== | ||
