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This protocol is a variant of the Hanahan protocol <cite>Hanahan91</cite> using CCMB80 buffer for DH10B and TOP10 strains. It builds on Example 2 of the [[Media:pat6855494.pdf | Bloom05 patent]] as well. This protocol has been tested only on TOP10 cells. See [[Bacterial Transformation]] for a more general discussion of other techniques. The [[Media:pat6960464.pdf | Jesse '464 patent]] describes using this buffer for DH5α cells. | This protocol is a variant of the Hanahan protocol <cite>Hanahan91</cite> using CCMB80 buffer for DH10B and TOP10 strains. It builds on Example 2 of the [[Media:pat6855494.pdf | Bloom05 patent]] as well. This protocol has been tested only on TOP10 cells. See [[Bacterial Transformation]] for a more general discussion of other techniques. The [[Media:pat6960464.pdf | Jesse '464 patent]] describes using this buffer for DH5α cells. The [[Media:pat6709852.pdf | Bloom04]] patent describes the use of essentially the same protocol for the Invitrogen Mach 1 cells. | ||
===Preparing glassware and media=== | ===Preparing glassware and media=== | ||
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<biblio> | <biblio> | ||
# Hanahan91 pmid=1943786 | # Hanahan91 pmid=1943786 | ||
# Bloom04 US Patent 6,709,852 [[Media:pat6709852.pdf]] | |||
# Bloom05 US Patent 6,855,494 [[Media:pat6855494.pdf]] | # Bloom05 US Patent 6,855,494 [[Media:pat6855494.pdf]] | ||
# Jesse05 US Patent 6,960,464 [[Media:pat6960464.pdf]] | # Jesse05 US Patent 6,960,464 [[Media:pat6960464.pdf]] |
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