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3. If your fresh broth is more turbid than the McFarland Standard, pipet one ml of the broth culture into a sterile glass tube and adjust the concentration of the cells by matching by eye the "cloudiness" (turbidity) of the cells in the glass tube to a standard provided by your instructor. <BR> | 3. If your fresh broth is more turbid than the McFarland Standard, pipet one ml of the broth culture into a sterile glass tube and adjust the concentration of the cells by matching by eye the "cloudiness" (turbidity) of the cells in the glass tube to a standard provided by your instructor. <BR> | ||
4) | 4) If your fresh broth is less turbid than the McFarland Standard, add bacteria from a freshly grown colony of the isolate grown on solid medium to concentrate the cells. <BR> | ||
The ending volume is irrelevant. Vortex to mix. <BR> | |||
[[Image:mcfarlandtable.jpg]]<BR> | [[Image:mcfarlandtable.jpg]]<BR> |
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