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Isolation of murine splenocytes: Difference between revisions
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Isolation of murine splenocytes (view source)
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==Overview== | ==Overview== | ||
In order to study spleen cells (e.g. lymphocytes, granulocytes, other immune cells), it helps to make single-cell suspensions so that the cells can be manipulated ex vivo easily. This protocol suggests ways in which you can do this without a lot of equipment or expensive supplies. This protocol can also be used to make cell suspensions from other lymphoid organs, such as the thymus or lymph nodes (see Current Protocols in Immunology, Unit 1.9 | In order to study spleen cells (e.g. lymphocytes, granulocytes, other immune cells), it helps to make single-cell suspensions so that the cells can be manipulated ex vivo easily. This protocol suggests ways in which you can do this without a lot of equipment or expensive supplies. This protocol can also be used to make cell suspensions from other lymphoid organs, such as the thymus or lymph nodes (see Current Protocols in Immunology, Unit 1.9 <cite>CP1</cite>). | ||
==Materials== | ==Materials== | ||
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<!-- If this protocol has papers or books associated with it, list those references here. See the [[OpenWetWare:Biblio]] page for more information. --> | <!-- If this protocol has papers or books associated with it, list those references here. See the [[OpenWetWare:Biblio]] page for more information. --> | ||
<biblio> | <biblio> | ||
# | #CP1 Current Protocols in Immunology, Unit 1.9: Removal of Lymphoid Organs [http://www.mrw.interscience.wiley.com/cp/cpim/articles/im0109/frame.html# link] (subscription required) | ||
# | #CP3 Current Protocols in Immunology, Unit 3.1: Isolation of Mouse Mononuclear Cells [http://www.mrw.interscience.wiley.com/cp/cpim/articles/im0301/frame.html link] (subscription required) | ||
</biblio> | </biblio> | ||
