Phusion: Difference between revisions

Phusion (view source)

23 bytes removed , 13 September 2006

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-'''What is it?'''
+==Description==
-• a Fusion of a Pyrococcus (Pfu) -like enzyme with a double-strand DNA binding domain → increased processivity.
+Fusion of a Pyrococcus (Pfu) -like enzyme with a double-strand DNA binding domain → increased processivity.
-'''Stats about Phusion™ High-Fidelity DNA Polymerase'''
+==Statistics about Phusion™ High-Fidelity DNA Polymerase==
+*10x processivity compared to Taq
-• 10x processivity compared to Taq
+*50x fidelity compared to Taq
+*Creates blunt end
-• 50x fidelity compared to Taq
+*Error rate is 4.4 X 10-7 in Phusion HF buffer and 9.5 X 10-7 in GC buffer
+*Non-displacing
-• creates blunt end
-• error rate is 4.4 X 10-7 in Phusion HF buffer and 9.5 X 10-7 in GC buffer
-• non-displacing
-'''Links'''
+==References==
 *[http://www.neb.com/nebecomm/products/productF-530.asp Purchase]
 *[http://www.finnzymes.fi/products/pcr/phusion_datasheet_f530sl_1_3_low.pdf Manual]