321
edits
Line 62: | Line 62: | ||
12. On the ImageJ interface, select the "magic wand" button and then click on the line defining the area of the curve of the first standard | 12. On the ImageJ interface, select the "magic wand" button and then click on the line defining the area of the curve of the first standard | ||
: [[Image:imagejmagicwand.jpg|300px]][[Image:imagejclickhere.jpg|300px]][[Image:clickherex3.jpg]] | : [[Image:imagejmagicwand.jpg|300px]][[Image:imagejclickhere.jpg|300px]][[Image:clickherex3.jpg|300px]] | ||
:- Continue selecting the area outlines of the remaining lanes | :- Continue selecting the area outlines of the remaining lanes | ||
:*The measurement of the areas will be bumped to a "Results" window | :*The measurement of the areas will be bumped to a "Results" window | ||
:*Be aware that ImageJ isn't "smart" -- The order you click | :*Be aware that ImageJ isn't "smart" -- The order in which you click corresponds to the numbers in the Results window; numbering does NOT correspond to the lanes you defined! | ||
:* If you click on the wrong part, you can | :*If you click on the wrong part of the histogram, you can find the bad measurement in the Results window, highlight it, go to '''Edit→cut''' and remove the bad entry. You should fix this as soon as you click on the wrong part of the histogram to keep your numbering correct. | ||
: [[Image:imagejarearesults.jpg|300px]] | : [[Image:imagejarearesults.jpg|300px]] | ||
edits