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CH391L/S13/QuorumSensing: Difference between revisions
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==Application to Synthetic Biology: Biopixels== | ==Application to Synthetic Biology: Biopixels== | ||
[[Image:biopixelalteration.png | Design of the threshold and period modulation circuits<cite>#Prindle</cite>. | thumb|left|200px]] | |||
[[Image:biopixelresult.png | Results of the period modulation (top) and threshold circuit (bottom)<cite>#Prindle</cite>. | thumb|left|200px]] | |||
[[Image:BiopixelsSystem.png | '''A.''' The LuxI/AiiA system that produces the H2O2 oscillations. Peach and Purple arrows represent activation of the promoters, while the red lines represents repression. LuxI and AiiA are responsible for AHL oscillations, ndh leads to production of H2O2, and sfGFP produces the reporter. '''B.''' Conceptual design of the microfluidic array<cite>#Prindle</cite>. | thumb|right|300px]] | |||
===Introduction=== | ===Introduction=== | ||
While quorum sensing is a robust system in the highly variable cellular environment, it is limited by the rate of diffusion and thus is a slow process that constrains the physical size of the system. Communication can also be achieved through gas phase signals, which are relatively faster than diffusion through liquid media, but also are weaker and short lived. Prindle et al. have combined these two signaling methods in order to create "biopixels", a large scale, yet robust quorum sensing system<cite>#Prindle</cite>. | While quorum sensing is a robust system in the highly variable cellular environment, it is limited by the rate of diffusion and thus is a slow process that constrains the physical size of the system. Communication can also be achieved through gas phase signals, which are relatively faster than diffusion through liquid media, but also are weaker and short lived. Prindle et al. have combined these two signaling methods in order to create "biopixels", a large scale, yet robust quorum sensing system<cite>#Prindle</cite>. | ||
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===Arsenic Sensor=== | ===Arsenic Sensor=== | ||
They added an additional copy of LuxI under the control of an arsenite-responsive promoter that is repressed in the absence of arsenite. When there is no arsenite, the system generates baseline oscillations. With arsenite, the additional LuxI promoter becomes active and increases the amplitude and period of oscillation. They found this system to be very sensitive and able to reliably sense concentrations of arsenite as low as 0.2 μM (WHO recommends 0.5 μM for developing nations). | They added an additional copy of LuxI under the control of an arsenite-responsive promoter that is repressed in the absence of arsenite. When there is no arsenite, the system generates baseline oscillations. With arsenite, the additional LuxI promoter becomes active and increases the amplitude and period of oscillation. They found this system to be very sensitive and able to reliably sense concentrations of arsenite as low as 0.2 μM (WHO recommends 0.5 μM for developing nations). | ||
