BISC110/S13: Series 1 Lab 1 Boot Camp: Difference between revisions

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BISC110/S13: Series 1 Lab 1 Boot Camp (view source)

Revision as of 09:06, 11 January 2013

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→‎PART II: Basic Laboratory Equipment
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'''Erlenmeyer Flasks''' are used to prepare solutions and microbiological media.  They range in size from 25mL to 6L.  Volume markings on an Erlenmeyer flask are only approximate and are not an accurate measure of volume.
'''Erlenmeyer Flasks''' are used to prepare solutions and microbiological media.  They range in size from 25mL to 6L.  Volume markings on an Erlenmeyer flask are only approximate and are not an accurate measure of volume.


'''Test tubes''' will be provided to you throughout the semester for experimentation.  Round bottom glass test tubes will be used for making dilutions and biochemical assays.  These tubes are identified by the diameter of the tube, so a 13mm test tube has a diameter of 13mm.  A 16mm tube has a diameter of 16mm and can hold a greater volume of liquid than the 13mm.  Centrifuge tubes can be glass or plastic, are often pointed at the bottom, and identified by the maximum volume they can contain.  They are used in a centrifuge to separate biologic materials.   
'''Tubes''' will be provided to you throughout the semester for experimentation.  Round bottom glass test tubes will be used for making dilutions and biochemical assays.  These tubes are identified by the diameter of the tube, so a 13mm test tube has a diameter of 13mm.  A 16mm tube has a diameter of 16mm and can hold a greater volume of liquid than the 13mm.  Centrifuge tubes can be glass or plastic, are often pointed at the bottom, and identified by the maximum volume they can contain.  They are used in a centrifuge to separate biologic materials.   


'''Graduated Cylinders''' are used to measure volumes from 10mL to 2L.  The gradations on the cylinder are an accurate measure of the volume of liquid contained in the cylinder. The liquid in the cylinder will form a meniscus as shown in the diagram below. To measure the proper volume, the bottom of the meniscus must rest on the desired volume mark on the cylinder.
'''Graduated Cylinders''' are used to measure volumes from 10mL to 2L.  The gradations on the cylinder are an accurate measure of the volume of liquid contained in the cylinder. The liquid in the cylinder will form a meniscus as shown in the diagram below. To measure the proper volume, the bottom of the meniscus must rest on the desired volume mark on the cylinder.
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