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BISC219/F12: RNAi Lab 7

23 bytes added, 09:02, 3 December 2012
Agarose Gel Electrophoresis
Load 15μL of each PCR product into separate wells in the gel. Make sure they are loaded in alphabetical order left to right, A, B, C. <BR>
Load 5 μL of the 100 base pair DNA ladder in the well on the far right. Our standards ladder is product number NEB323-2S from New England Biologicals. The manufacturer supplies the following information about this product.<BR>
[[Image:NEB323-S1.jpg]]
<BR>
Run the gel for ~ 20min. at ~120 volts. <BR>
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