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Tucker Crum (talk | contribs) |
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Load 15μL of each PCR product into separate wells in the gel. Make sure they are loaded in alphabetical order left to right, A, B, C. <BR> | Load 15μL of each PCR product into separate wells in the gel. Make sure they are loaded in alphabetical order left to right, A, B, C. <BR> | ||
Load 5 μL of the 100 base pair DNA ladder in the well on the far right. Our standards ladder is product number NEB323-2S from New England Biologicals. The manufacturer supplies the following information about this product.<BR> | Load 5 μL of the 100 base pair DNA ladder in the well on the far right. Our standards ladder is product number NEB323-2S from New England Biologicals. The manufacturer supplies the following information about this product.<BR> | ||
[[Image:NEB323-S1.jpg]] | |||
<BR> | <BR> | ||
Run the gel for ~ 20min. at ~120 volts. <BR> | Run the gel for ~ 20min. at ~120 volts. <BR> |
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