289
edits
IGEM:Paris Bettencourt 2012/Previous Biosafety iGEM Projects: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
IGEM:Paris Bettencourt 2012/Previous Biosafety iGEM Projects (view source)
Revision as of 16:21, 11 September 2012
, 11 September 2012no edit summary
mNo edit summary |
No edit summary |
||
| Line 26: | Line 26: | ||
| '''''Kill switch.''''' "Our kill switch is designed by inserting an antimicrobial peptide (AMP) gene into E.Coli" | | '''''Kill switch.''''' "Our kill switch is designed by inserting an antimicrobial peptide (AMP) gene into E.Coli" | ||
<p style="text-align:right;"> [http://2011.igem.org/Team:St_Andrews/biosafe Read More] </p> | <p style="text-align:right;"> [http://2011.igem.org/Team:St_Andrews/biosafe Read More] </p> | ||
| Used the LIVE/DEAD Baclight Bacterial Viability kit, but don't have any quantitative results in terms of cell death. "The relationship between the concentration of arabinose and the amount and rate of cell death seems linear in nature." | | Used the LIVE/DEAD Baclight Bacterial Viability kit, but don't have any quantitative results in terms of number or proportion of cell death. "The relationship between the concentration of arabinose and the amount and rate of cell death seems linear in nature." | ||
|- | |- | ||
| Line 38: | Line 38: | ||
*Once pores are formed by holin, lysozyme can access the periplasmic space and degrade the cell wall, causing cell lysis. | *Once pores are formed by holin, lysozyme can access the periplasmic space and degrade the cell wall, causing cell lysis. | ||
<p style="text-align:right;"> [http://2011.igem.org/Team:Imperial_College_London/Project_Gene_Overview Read More] </p> | <p style="text-align:right;"> [http://2011.igem.org/Team:Imperial_College_London/Project_Gene_Overview Read More] </p> | ||
| Anti-holin was expressed in cells, but no experiments for this system have been made. "It seems very likely that our GM E. coli have been able to survive in soil and retain their plasmid for six weeks despite competition and selective pressure against the plasmid." | |||
|- | |- | ||
| Line 47: | Line 48: | ||
| '''''Encapsulation in a gel.''''' We encapsulated our bacteria in beeds made out of a non toxic gel. | | '''''Encapsulation in a gel.''''' We encapsulated our bacteria in beeds made out of a non toxic gel. | ||
<p style="text-align:right;"> [http://2010.igem.org/Team:BCCS-Bristol/Wetlab/Safety Read More] </p> | <p style="text-align:right;"> [http://2010.igem.org/Team:BCCS-Bristol/Wetlab/Safety Read More] </p> | ||
| "Bacteria are kept separate from the environment, reducing public safety fears." No quantification provided. | |||
|- | |- | ||
