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#Repeat for colony B
#Snap the lid on the tubes, pulse them briefly in the microcentrifuge with the appropriate rotor and adapter and bring them to the thermal cycler for PCR initiation.
#Finally, seal the plate with parafilm. Invert it (agar side up) and store it in the refrigerator in your lab day's rack until the day before the next lab when you will set up an overnight culture from THIS a colonythat is positive for ''lys-2''.


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