504
edits
Corum:DNA Hybridization: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
m
no edit summary
Sean P Corum (talk | contribs) m (→Materials) |
Sean P Corum (talk | contribs) mNo edit summary |
||
| Line 1: | Line 1: | ||
==Overview== | ==Overview== | ||
Hybridization of ssDNA oligonucleotides. | Hybridization of ssDNA oligonucleotides. | ||
| Line 22: | Line 20: | ||
#* 2 μL 10μM linker + 198 μL H<sub>2</sub>O = 200 μL 100nM linker | #* 2 μL 10μM linker + 198 μL H<sub>2</sub>O = 200 μL 100nM linker | ||
# Store 1mM, 10μM, and 100nM stocks at -40 °C in latest "Primer" box. Keep an aliquot of the 100nM linker in your own box for use in constructions. | # Store 1mM, 10μM, and 100nM stocks at -40 °C in latest "Primer" box. Keep an aliquot of the 100nM linker in your own box for use in constructions. | ||
==Signature== | |||
*'''SC 19:27, 24 July 2012 (EDT)''': | |||
==Notes== | ==Notes== | ||
| Line 30: | Line 31: | ||
Please sign your name to your note by adding <font face="courier"><nowiki>'''*~~~~''':</nowiki></font> to the beginning of your tip. | Please sign your name to your note by adding <font face="courier"><nowiki>'''*~~~~''':</nowiki></font> to the beginning of your tip. | ||
==Contact== | ==Contact== | ||
