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A ''biomolecular breadboard'' is a system that is designed to allow certain features of a circuit to be tested in a carefully controlled setting. These breadboards can be used to implement, debug, and characterize a wide variety of circuits, including both ''in vivo'' and ''in vitro'' devices. This page contains an overview of different biomolecular breadboards that are available. | A ''biomolecular breadboard'' is a system that is designed to allow certain features of a circuit to be tested in a carefully controlled setting. These breadboards can be used to implement, debug, and characterize a wide variety of circuits, including both ''in vivo'' and ''in vitro'' devices. This page contains an overview of different biomolecular breadboards that are available. | ||
=== Breadboard overview === | |||
[[Image:breadboard-process.png]] | |||
The figure above provides an overview of the basic breadboarding process. At the left is a circuit that we wish to implement and transform into a cell or other bimolecular chassis. Rather than try to direct get the circuit working in the cell, which requires time consuming iterations and difficult debugging, we instead use a sequence of simpler test environments ("breadboards"), where we can do much more rapid iterations between experiments, modeling and design. | |||
=== Cell-free circuit breadboard === | === Cell-free circuit breadboard === | ||
The cell-free circuit breadboard family is a sequence of ''in vitro'' protocols that can be used to test transcription and translation (TX-TL) circuits in a set of systematically-constructed environments that explore different elements of the external conditions in which the circuits must operate. This breadboard is based on the work of Vincent Noireaux at U. Minnesota. | The cell-free circuit breadboard family is a sequence of ''in vitro'' protocols that can be used to test transcription and translation (TX-TL) circuits in a set of systematically-constructed environments that explore different elements of the external conditions in which the circuits must operate. This breadboard is based on the work of Vincent Noireaux at U. Minnesota. | ||
=== DNA origami breadboard === | |||
=== Artificial cells === | |||
=== Biochemical wires === |
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