IGEM:Paris Bettencourt 2012/Previous Biosafety iGEM Projects: Difference between revisions

  |# Water filtration system for containment of microbes (filters all microbes, does not retain free DNA).

# Use the ccdB gene (BBa_P1016 and BBa_P1010)on a plasmid in conjunction with an E. coli strain such as DB3.1 (BBa_V1005). The plasmid will code for the “death gene” which will kill any cell that does not code for immunity in its genome. If a native microorganism would uptake this man-made plasmid, it would die, preventing the propagation of the recombinant DNA in the environment (Featured Parts: Cell Death).

# Another similar “suicide” containment system uses streptavidin (BBa_J36841) (Kaplan, Mello et al. 1999; Urgun-Demirtas, Stark et al. 2006). This protein binds very tightly to biotin, a required co-enzyme for many metabolic pathways. This makes biotin unavailable and causes cell death. Kaplan et al. reported cell counts were reduced 99.9% in eight hours after their system was activated by absence of pollutant to degrade.

  <p style="text-align:right;"> [http://2011.igem.org/Team:ULB-Brussels/removing Read More] </p>