771
edits
Miniprep/TENS miniprep: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
m
no edit summary
m (New page: {{back to protocols}} ==Background== TENS method of minipreps. Fast but dirty. ==Protocol== #Transfer 1.5 mL of an overnight culture containing your plasmid to an eppendorf tube and spin ...) |
mNo edit summary |
||
| Line 25: | Line 25: | ||
===TE=== | ===TE=== | ||
10 mM Tris-HCl, pH 8 | :10 mM Tris-HCl, pH 8 | ||
1 mM EDTA | :1 mM EDTA | ||
==TENS== | ===TENS=== | ||
(500 mL recipe) | (500 mL recipe) | ||
:5 mL 1M Tris-HCl pH 8.0 | |||
:12.5 mL 20% SDS | |||
:5 mL 10N NaOH | |||
:Enough H20 to 500 mL | |||
[[Category:Protocol]] | [[Category:Protocol]] | ||
