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===Green Fluorescent Protein=== | ===Green Fluorescent Protein=== | ||
[[Image:Green_Fluroescent_Mice.jpg|thumb|left|Mice transfected with GFP. One can easily distinguish the wild-type mouse (middle) from the two mice with GFP (left and right)<cite>Moen2011</cite>.]] | [[Image:Green_Fluroescent_Mice.jpg|thumb|left|Mice transfected with GFP. One can easily distinguish the wild-type mouse (middle) from the two mice with GFP (left and right)<cite>Moen2011</cite>.]] | ||
Green Fluorescent Protein, or GFP, was first isolated from the crystal jellyfish Aequorea victoria in | Green Fluorescent Protein, or GFP, was first isolated from the crystal jellyfish Aequorea victoria in the 1960s. In 1994, GFP was successfully cloned<cite>Chalfie1994</cite>, allowing researchers to use the protein as a screenable marker for the first time. Virtually harmless in live cells, GFP has the unique pheotype of glowing bright green under ultraviolet light. GFP functions entirely of its own accord, and requires no exogenous material besides ionizing radiation in order to fluoresce. This allows GFP to be used as a marker accompanying transfected DNA, and has been used extensively in academia. | ||
In 2011, | |||
==Artificial Selection== | ==Artificial Selection== | ||
==Errors and Issues== | ==Errors and Issues== | ||
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#Moen2011 pmid=22251838 | #Moen2011 pmid=22251838 | ||
//Observes tumor growth in mice by introducing GFP into the mice. | //Observes tumor growth in mice by introducing GFP into the mice. | ||
#Chalfie1994 pmid=8303295 | |||
//The first instance of using GFP as a marker. | |||
</biblio> | </biblio> | ||
