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==Selectable Markers Overview== | ==Selectable Markers Overview== | ||
[[Image:Antibiotic Resistance Markers.jpg|thumb|right|Example application of selectable genetic markers in nematodes. Only nematodes transfected with markers survive and proliferate.]] | [[Image:Antibiotic Resistance Markers.jpg|thumb|right|Example application of selectable genetic markers in nematodes. Only nematodes transfected with markers survive and proliferate<cite>Giordano-Santini2011</cite>.]] | ||
Selectable genetic markers are extraneous genes that are introduced into a cell, conferring a previously absent resistance. These markers are primarily used to "mark" the successful transformation of DNA into a plasmid. Oftentimes, selectable markers are accompanied by other extraneous genes that is the primary gene of interest; the marker simply serves to distinguish between successful transformations, and unaltered cells. It is not atypical to witness transformation efficiencies as low as .05%, making it difficult to pick correct cellular colonies without additional techniques. | Selectable genetic markers are extraneous genes that are introduced into a cell, conferring a previously absent resistance. These markers are primarily used to "mark" the successful transformation of DNA into a plasmid. Oftentimes, selectable markers are accompanied by other extraneous genes that is the primary gene of interest; the marker simply serves to distinguish between successful transformations, and unaltered cells. It is not atypical to witness transformation efficiencies as low as .05%, making it difficult to pick correct cellular colonies without additional techniques. | ||
