146
edits
CH391L/S12/Selectablegeneticmarkers: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
CH391L/S12/Selectablegeneticmarkers (view source)
Revision as of 15:19, 19 February 2012
, 19 February 2012no edit summary
No edit summary |
No edit summary |
||
| Line 2: | Line 2: | ||
==Selectable Markers Overview== | ==Selectable Markers Overview== | ||
[[Image:Antibiotic Resistance Markers.jpg|thumb|right|Example application of selectable genetic markers in nematodes. Only nematodes transfected with markers survive and proliferate.]] | |||
Selectable genetic markers are extraneous genes that are introduced into a cell, conferring a previously absent resistance. These markers are primarily used to "mark" the successful transformation of DNA into a plasmid. Oftentimes, selectable markers are accompanied by other extraneous genes that is the primary gene of interest; the marker simply serves to distinguish between successful transformations, and unaltered cells. It is not atypical to witness transformation efficiencies as low as .05%, making it difficult to pick correct cellular colonies without additional techniques. | Selectable genetic markers are extraneous genes that are introduced into a cell, conferring a previously absent resistance. These markers are primarily used to "mark" the successful transformation of DNA into a plasmid. Oftentimes, selectable markers are accompanied by other extraneous genes that is the primary gene of interest; the marker simply serves to distinguish between successful transformations, and unaltered cells. It is not atypical to witness transformation efficiencies as low as .05%, making it difficult to pick correct cellular colonies without additional techniques. | ||
| Line 16: | Line 18: | ||
==References== | ==References== | ||
<biblio> | <biblio> | ||
#Giordano-Santini2011 pmid=21431833 | |||
//Review article about selectable genetic markers as used in nematodes. Relatively new field for nematodes, possible due to the completion of the <i>Caenorhabditis elegans</i> genome. | |||
</biblio> | </biblio> | ||
