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[[Category:CH391L_S12]] | [[Category:CH391L_S12]] | ||
==Markers Overview== | ==Selectable Markers Overview== | ||
Selectable genetic markers are extraneous genes that are introduced into a cell, conferring a previously absent resistance. These markers are primarily used to "mark" the successful transformation of DNA into a plasmid. Oftentimes, selectable markers are accompanied by other extraneous genes that is the primary gene of interest; the marker simply serves to distinguish between successful transformations, and unaltered cells. It is not atypical to witness transformation efficiencies as low as .05%, making it difficult to pick correct cellular colonies without additional techniques. | |||
For instance, selectable genetic markers can be used to confer ampicillin resistance to E. coli. These newly resistant E. coli can then be grown on culture plates with ampicillin, allowing only E.coli with successfully transformed DNA to proliferate. | |||
selectable | selectable | ||
screenable | screenable | ||
==Types of Markers== | ==Types of Selectable Markers== | ||
==Screening== | ==Screening== | ||
blue/white | blue/white | ||
