Our goal is to upregulate production of dsRNA of our worm gene of interest in pL4440 vector plasmids containing that gene in HT115(DE3) bacteria. <br><bR>
'''To induce your cultures:''' <br>#
Add 5 μL of 0.5 M IPTG to your culture. What is the effective concentration of IPTG in your culture?
#Put your culture back in the 37°C incubator in the spinning wheel for approximately 3-4 hours.
After the lab introduction, we will head to a more comfortable room to discuss the papers you were assigned.
'''To do after induction is complete:'''
'''4 days before next lab:'''
#Come into lab and find your stack of plates. For Friday, Monday and Tuesday sections your plates will be at room temperature by your worm picks. For Wednesday and Thursday sections your plates will be in the
white refrigerator in the lab - please warm the plates in your hands for a few minutes or you will shock the worms with the cold.
#On 2 of the experimental plates add 2 L4 wild type (N2) hermaphrodites
#On 2 of the experimental plates add 2 L4 ''rrf-3'' hermaphrodites