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BISC219/F11: RNAi Lab 9
Revision as of 10:59, 8 November 2011
295 bytes added
10:59, 8 November 2011
→Lab 9: Series 3 Reverse Genetics-Induction of HT115(DE3) feeding strains for RNAi
'''4 days before next lab:'''
#Come into lab and find your stack of plates
. For Friday, Monday and Tuesday sections your plates will be at room temperature by your worm picks. For Wednesday and Thursday sections your plates will be in the white refrigerator in the lab - please warm the plates in your hands for a few minutes or you will shock the worms with the cold
#On 2 of the experimental plates add 2 L4 wild type (N2) hermaphrodites
#On 2 of the experimental plates add 2 L4 ''rrf-3'' hermaphrodites
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