1,973
edits
TOP10 chemically competent cells: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
→Measurement of competence
| Line 56: | Line 56: | ||
* Plate 20 μl on AMP plates using 3.5 mm glass beads | * Plate 20 μl on AMP plates using 3.5 mm glass beads | ||
* Transformation efficiency is 15 x colony count x 10<sup>5</sup> | * Transformation efficiency is 15 x colony count x 10<sup>5</sup> | ||
* There is anecdotal evidence that refreezing partially used cell aliquots dramatically reduces transformation efficiency. | |||
==References== | ==References== | ||
