TOP10 chemically competent cells: Difference between revisions

TOP10 chemically competent cells (view source)

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	This protocol is a variant of the Hanahan protocol <cite>Hanahan91</cite> using CCMB80 buffer for DH10B and TOP10 strains. It builds on Example 2 of the [[Media:pat6855494.pdf \| Bloom05 patent]] as well.		This protocol is a variant of the Hanahan protocol <cite>Hanahan91</cite> using CCMB80 buffer for DH10B and TOP10 strains. It builds on Example 2 of the [[Media:pat6855494.pdf \| Bloom05 patent]] as well. This protocol has been tested only on TOP10 cells. See [[Bacterial Transformation]] for a more general discussion of other techniques.

	===Preparing glassware and media===		===Preparing glassware and media===