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Lactococcus transformation: Difference between revisions
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6. Centrifuge cells for 15 mins at 3000g. <br> | 6. Centrifuge cells for 15 mins at 3000g. <br> | ||
7. Resuspend pellet in 500µl Electroporation Buffer (0.5M Sucrose, 10% glycerol). <br> | 7. Resuspend pellet in 500µl Electroporation Buffer (0.5M Sucrose, 10% glycerol). <br> | ||
8. Separate into 100µl aliquots and store at -80°C until use. <br> | 8. Separate into 100µl aliquots and store at -80°C until use. <br> <br> | ||
===Electro-Transformation=== | ===Electro-Transformation=== | ||
