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Sauer:bis-Tris SDS-PAGE, the very best: Difference between revisions
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Sauer:bis-Tris SDS-PAGE, the very best (view source)
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The Stacking and Resolving layers of the gel use the same buffer. This allows gels to be cast and stored for a long time (diffusion doesn't ruin the stacking chemistry). Also, the same tank running buffer is used at both the cathode and anode. | The Stacking and Resolving layers of the gel use the same buffer. This allows gels to be cast and stored for a long time (diffusion doesn't ruin the stacking chemistry). Also, the same tank running buffer is used at both the cathode and anode. | ||
[[Image:SDM_gel1.jpg]] | [[Image:SDM_gel1.jpg]] [[Image:SDM_gel2.jpg]] | ||
'''Sample bis-Tris MES-Tris Gels''' The first gel is of purified proteins. The second gel is of total E. coli lysate. Note the broad separation range of the molecular weight markers. | |||
<u>'''Materials'''</u> | <u>'''Materials'''</u> | ||
