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1,756 bytes added ,  21:34, 3 March 2010
add homebrew taq
: Cathal Garvey: ''I created a protocol to cook up lab-quality growth media using only off-the-shelf ingredients, most or all of which should be available at a pharmacy and/or health store. The only ingredient not available in ready-to-use form is the peptone that most media call for; I include a recipe and super-easy instructions on how to make peptone from Soy (called Phytone) or Casein (similar to Tryptone) using the enzyme Bromelain, which is widely available as a digestive aid or as meat tenderiser and performs quite well at a variety of temperatures and pH conditions.'' and
= Homebrew Taq =
Purification and characterization of Taq polymerase: A 9-week
biochemistry laboratory project for undergraduate students 10.1002/bmb.20352
Robert M. Bellin *, Mary K. Bruno a, Melissa A. Farrow
Department of Biology, College of the Holy Cross, Worcester, Massachusetts 01610
email: Robert M. Bellin (
We have developed a 9-week undergraduate laboratory series focused on
the purification and characterization of Thermus aquaticus DNA
polymerase (Taq). Our aim was to provide undergraduate biochemistry
students with a full-semester continuing project simulating a
research-like experience, while having each week's procedure focus on
a single learning goal. The laboratory series has been taught for the
past 7 years, and survey-based assessment of the effectiveness of the
laboratory series was completed during the 2006 and 2007 fall
semesters. Statistical analysis of the survey results demonstrate that
the laboratory series is very effective in teaching students the
theory and practice of protein purification and analysis while also
demonstrating positive results in more broad areas of scientific skill
and knowledge. Amongst the findings, the largest reported increases in
knowledge were related to students' understanding of how patent law
relates to laboratory science, a topic of great importance to modern
researchers that is readily discussed in relation to Taq polymerase.
Overall, this laboratory series proves to be a very effective
component in the curricula of undergraduate biology and chemistry
majors and may be an appropriate laboratory experience for

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