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Tucker Crum (talk | contribs) |
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<UL><LI> | <UL><LI> | ||
'''Enrichment:'''<BR> | '''Enrichment:'''<BR> | ||
Measure 1 g of soil and put it in a 5ml tube of 50microgram/ml cycloheximide. Mix well <LI> | |||
Label a plate of '''Cytophagia agar'''<LI> | Label a plate of '''Cytophagia agar'''<LI> | ||
Using a flame sterilized inoculating loop, streak 3 fine lines of the | Using a flame sterilized inoculating loop, streak 3 fine lines of the soil/cycloheximide slurry across the plate as illustrated below:<BR> | ||
[[image:GlidingStreakPlate.jpg]]<BR> | [[image:GlidingStreakPlate.jpg]]<BR> | ||
Incubate the plate at room temperature.<LI> | Incubate the plate at room temperature.<LI> | ||
Examine the plates every few days. Use a dissecting scope or a strong magnifying glass to detect the development of colored colonies that appear to be gliding out of the steaks and across the agar plate. To avoid selecting other types of motile bacteria, look for a pattern of fine flamelike projections at the edge of the swarm. Most flagellate colonies have an edge that is entire or coarsely lobed. <LI> | Examine the plates every few days. Use a dissecting scope or a strong magnifying glass to detect the development of colored colonies or colonies that appear to be gliding out of the steaks and across the agar plate. To avoid selecting other types of motile bacteria, look for a pattern of fine flamelike projections at the edge of the swarm. Most flagellate colonies have an edge that is entire or coarsely lobed. You will need to observe this plate for these bacteria to develop anywhere from 24 hours to 2 weeks. <LI> | ||
Make a wet mount from the colored or spreading colones or prepare a hanging drop slide for viewing with your brightfield microscope. Look for rod shaped bacteria with tapering or rounded ends. <LI> | Make a wet mount from the colored or spreading colones or prepare a hanging drop slide for viewing with your brightfield microscope. Look for rod shaped bacteria with tapering or rounded ends. <LI> | ||
'''Isolation:''' <BR> | '''Isolation:''' <BR> |
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