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BISC110: Series 4 Lab 11 Enzymology: Difference between revisions
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BISC110: Series 4 Lab 11 Enzymology (view source)
Revision as of 12:40, 3 December 2009
, 3 December 2009→Procedure for Invertase Assays
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15. Carefully remove your tubes from the hot water bath. There are test tube holders near the bath to help you with the hot tubes. CAUTION: Take care not to burn your fingers in the hot water or with the steam, and please check that you are removing your own tubes from the bath. | 15. Carefully remove your tubes from the hot water bath. There are test tube holders near the bath to help you with the hot tubes. CAUTION: Take care not to burn your fingers in the hot water or with the steam, and please check that you are removing your own tubes from the bath. | ||
16. Let your tubes sit on the bench for | 16. Let your tubes sit on the bench for 5min until they are cool to the touch. Transfer the contents (just pour them) into a new set of tubes. Then spin tubes 5–8 for 2min in a clinical centrifuge. The cells in these tubes must pellet completely and the solution must not look cloudy after this spin. DO NOT discard the supernatant after this spin. | ||
17. Wipe the outside of each test tube with a Kimwipe™ | 17. Wipe the outside of each test tube with a Kimwipe™. Using tube #1 to blank the spectrophotometer, read the absorbance of each reaction at 540nm and record all data in your lab notebook. Were the activity levels of the invertase produced by the yeast cells higher or lower than the activity of the purified invertase? | ||
''' Laboratory Clean up ''' | ''' Laboratory Clean up ''' | ||
