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Payne Lab: Difference between revisions
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→New methods for live cell imaging: Fluorescence microscopy and nanoparticle delivery.
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==='''New methods for live cell imaging: Fluorescence microscopy and nanoparticle delivery.'''=== | ==='''New methods for live cell imaging: Fluorescence microscopy and nanoparticle delivery.'''=== | ||
'''Intracellular delivery of nanoparticles.''' | |||
Nanoparticles have important biomedical applications ranging from the treatment of human disease with gene therapy to understanding basic cellular functions with fluorescent probes. For these applications to be fully realized it is necessary to deliver nanoparticles across the plasma membrane and into the cytosol of living cells. The Payne Lab is developing novel methods for the cytosolic delivery and targeting of nanoparticles in conjunction with the use of advanced microscopy techniques to understand the mechanisms of nanoparticle delivery. | |||
The Payne Lab is developing new optical techniques for | '''Fluorescence microscopy in challenging environments.''' | ||
While recent developments in fluorescence microscopy make it possible to image many of the dynamic events that are essential to cellular function, new methods are necessary to observe the dynamics of single molecules inside living cells. Imaging within live cells is difficult as the emission from fluorescent probes competes with the autofluorescence of the cell. The Payne Lab is developing new optical techniques for quantitative cellular imaging. Optical methods of interest include nanometer-level imaging, spectroscopic single-particle tracking, and multiphoton total internal reflection microscopy. | |||
