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SOB: Difference between revisions
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*10 mM NaCl | *10 mM NaCl | ||
*2.5 mM KCl | *2.5 mM KCl | ||
*20 mM MgSO<sub>4</sub> | *20 mM MgSO<sub>4</sub> | ||
Per liter: | |||
*5 g yeast extract | |||
*20 g tryptone | |||
*0.584 g NaCl | |||
*0.186 g KCl | |||
*2.4 g MgSO<sub>4</sub> | |||
''Note:'' Some formulations of SOB also include 10 mM MgCl<sub>2</sub>. | |||
==Source== | ==Source== | ||
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F. Ausubel et al., ''Short Protocols in Molecular Biology'' (John Wiley & Sons, ed. 4, 1999) pg. A1-36 | F. Ausubel et al., ''Short Protocols in Molecular Biology'' (John Wiley & Sons, ed. 4, 1999) pg. A1-36 | ||
==Trivia== | |||
SOB supposedly stands for super optimal broth. | |||
