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TE: Difference between revisions
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*Some protocols use TE 10:0.1 with 0.1 mM EDTA to reduce the interaction of the EDTA with downstream applications. | *Some protocols use TE 10:0.1 with 0.1 mM EDTA to reduce the interaction of the EDTA with downstream applications. | ||
*Some people use TE buffers with different pH's for different applications. For example, DNA is stored at pH 8 to reduce depurination, which is acid catalyzed, while RNA is stored at a slightly lower pH (7.5) because degradation of RNA is base-catalyzed. Most downstream reactions will not be influenced by the slightly different pH storage conditions. | *Some people use TE buffers with different pH's for different applications. For example, DNA is stored at pH 8 to reduce depurination, which is acid catalyzed, while RNA is stored at a slightly lower pH (7.5) because degradation of RNA is base-catalyzed. Most downstream reactions will not be influenced by the slightly different pH storage conditions. | ||
*For dilalution of primers Water for Injection(water that is used for dialute injections)can be used effectively rather than going for TE and also for PCR as well | *For dilalution of primers Water for Injection(water that is used for dialute injections)can be used effectively rather than going for TE and also for PCR as well. | ||
[[Category:Material]] and [[Category:Buffers]] | [[Category:Material]] and [[Category:Buffers]] | ||
