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Bacterial cell culture: Difference between revisions
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(new protocol for beginners to in vivo work, please supplement) |
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#Streak an [[Pour plates|agar plate]] from glyerol stock. Incubate plates overnight in the 37 °C room. | #Streak an [[Pour plates|agar plate]] from glyerol stock. Incubate plates overnight in the 37 °C room. | ||
#Take your plates from the warm room. Take an aliquot of the [Endy:Preparing Antibiotic Stocks|antibiotic](s) needed from the freezer, and set it on the bench to thaw. | #Take your plates from the warm room. Take an aliquot of the [[Endy:Preparing Antibiotic Stocks|antibiotic]](s) needed from the freezer, and set it on the bench to thaw. | ||
#Flame a glass pipette, open the bottle of medium and flame the mouth, measure out the amount you need to fill your tubes, flame the cap and recap the bottle as quickly as possible. | #Flame a glass pipette, open the bottle of medium and flame the mouth, measure out the amount you need to fill your tubes, flame the cap and recap the bottle as quickly as possible. | ||
#Remove the tube cap, flame the top of the culture tube, pipette in 5 ml, flame the top of the tube, and cap it. | #Remove the tube cap, flame the top of the culture tube, pipette in 5 ml, flame the top of the tube, and cap it. | ||
