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Polyacrylamide gel electrophoresis: Difference between revisions
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*For thicker gels (bigger wells) more gel will need to be made. The amount of gel necessary can be calculated volumetrically. | *For thicker gels (bigger wells) more gel will need to be made. The amount of gel necessary can be calculated volumetrically. | ||
*The voltage applied to the gel will vary according the the tube of gel you cast. The specified voltage is 1-8 volts/cm. Centimeters in this case specifies the length of the gel from top to bottom (i.e the direction the DNA will travel). | *The voltage applied to the gel will vary according the the tube of gel you cast. The specified voltage is 1-8 volts/cm. Centimeters in this case specifies the length of the gel from top to bottom (i.e the direction the DNA will travel). | ||
*To determine the | *To determine the final acrylamide concentration for your application use the following table: | ||
<center> | |||
{| border="1" | |||
|+ | |||
! Acrylamide Concentration(%) !! Optimal DNA Resolution (bp) | |||
|- | |||
| 3.5 || 1000-2000 | |||
|- | |||
| 5.0 || 80-500 | |||
|- | |||
| 8.0|| 60-400 | |||
|- | |||
| 12.0 || 40-200 | |||
|- | |||
| 15.0 || 25-150 | |||
|- | |||
| 20.0 || 6-100 | |||
|} | |||
</center> | |||
==Acknowledgments== | ==Acknowledgments== | ||
