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Polyacrylamide gel electrophoresis: Difference between revisions
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[[Image:Vertical_electrophoresis.JPG|300px|right]] | [[Image:Vertical_electrophoresis.JPG|300px|right]] | ||
*It is important to get the fill level right in the electrophoresis apparatus (please see the figure to the right) | *It is important to get the fill level right in the electrophoresis apparatus (please see the figure to the right). | ||
*For thicker gels (bigger wells) more gel will need to be made. The amount of gel necessary can be calculated volumetrically. | *For thicker gels (bigger wells) more gel will need to be made. The amount of gel necessary can be calculated volumetrically. | ||
*The voltage applied to the gel will vary according the the tube of gel you cast. The specified voltage is 1-8 volts/cm. Centimeters in this case specifies the length of the gel from top to bottom (i.e the direction the DNA will travel). | *The voltage applied to the gel will vary according the the tube of gel you cast. The specified voltage is 1-8 volts/cm. Centimeters in this case specifies the length of the gel from top to bottom (i.e the direction the DNA will travel). | ||
