1,721
edits
Richard Lab:Restriction Digest: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
→Materials
No edit summary |
|||
| Line 6: | Line 6: | ||
* Prepared DNA from miniprep, PCR, or Gel Extraction | * Prepared DNA from miniprep, PCR, or Gel Extraction | ||
* Restriction Endonucleases | * Restriction Endonucleases | ||
** With corresponding 10X buffer. | ** With corresponding 10X buffer. NEBuffer 2 can be used for most applications but check [http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/buffer_activity_restriction_enzymes.asp? this chart] to be sure. | ||
* BSA (optional) | * BSA (optional) | ||
* Phosphatase | * Phosphatase | ||
*Distilled water | |||
==Procedure== | ==Procedure== | ||
